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rosettesep human cd4+ t cell enrichment cocktail stemcell 15062  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rosettesep human cd4+ t cell enrichment cocktail stemcell 15062
    Rosettesep Human Cd4+ T Cell Enrichment Cocktail Stemcell 15062, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosettesep+human+cd4++t+cell+enrichment+cocktail+stemcell+15062/rosettesep+human+b+cell+enrichment+cocktail/pm38225245-234-23-30
    Average 90 stars, based on 1 article reviews
    rosettesep human cd4+ t cell enrichment cocktail stemcell 15062 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Selection:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Centrifugation:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Purification:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    FACS:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Knock-Out:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Gradient Centrifugation:

    Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
    Article Snippet: RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies, Catalog #15062) was then added to the blood sample to deplete all non-CD4 + T cells.

    Article Title: Antibodies against glucocorticoid-induced tumor necrosis factor receptor (GITR) and uses thereof
    Article Snippet: For experiments with CD4+ T cells, CD4+T cells were obtained from human PBMCs (donors 1 and 2) with RosetteSep Human CD4+ T cell enrichment cocktail (StemCell Technology #15062) according to the manufacturer's protocol.

    Article Title: Tonic-signaling chimeric antigen receptors drive human regulatory T cell exhaustion.
    Article Snippet: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies, 07861).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis
    Article Snippet: Primary human CD4 + T cells were isolated from leukocyte cones using the RosetteSep Human CD4 + T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.
    Article Snippet: ADAM10 knock-out in primary CD4+ T cells, cell expansion and isolation Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies 15062).

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines.
    Article Snippet: Purification of T-cell subgroups from blood, cell sorting, and culturing with and measuring cytokines HumanCD4+ T cells were isolated from ~2 × 108 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.

    Article Title: Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance.
    Article Snippet: CD4, CD8 and NK cells were isolated by density gradient centrifugation with lymphoprep and SepMateTM-50 (IVD) tubes following the addition of RosetteSep Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15062), CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies, 15063) and NK Cell Enrichment Cocktail (STEMCELL Technologies, 15065) respectively.

    Article Title: Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
    Article Snippet: Human CD4 + T cells were isolated from ~2 × 10 8 elutriated lymphocytes to ~90% purity by negative selection using RosetteSep human CD4 + T cell enrichment cocktail (Cat# 15062, Stem Cell Technologies), using Ficoll/Hypaque (Cat# 45-001-749, Cytiva) density centrifugation.



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